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the controlled expression of many genes, including g protein coupled receptors (gpcrs), is important for delineating gene functions in complex model systems. binary systems for inducible regulation of transgene expression are widely used in mice. one system is the tta/tre expression system, composed of a tetracycline dependent dna binding factor and a separate tetracycline operon. however, the
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to assay for expression and localization of neural cell adhesion molecule (ncam) and polysialic acid (polysia) in the chick cornea during embryonic and postnatal development. kwa b, springall
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the cornea is the most densely innervated and sensitive tissue on the surface of the body. during early chick embryonic development, corneal nerves are derived from neural crest cells located in the ophthalmic lobe of the trigeminal ganglion. 1 –4 trigeminal nerve axon fascicles reach the corneal periphery by embryonic day (e)5. the nerves are repelled from entering the cornea and form a
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drops of ninhydrin solution (10 mg of ninhydrine in 200 ml of acetone) are added to 2 ml of aqueous filtrate and observed for the present of characteristic purple colour. was recorded a biuret test (gahan 1984): an aliquot of 2 ml of filtrate is treated with one drop of 2% copper sulphate solution. to
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the cornea is the most densely innervated and sensitive tissue on the surface of the body. during early chick embryonic development, corneal nerves are derived from neural crest cells located in the ophthalmic lobe of the trigeminal ganglion. 1 –4 trigeminal nerve axon fascicles reach the corneal periphery by embryonic day (e)5. the nerves are repelled from entering the cornea and form a
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